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    订购信息
    上海豆奶视频在线观看生物科技公司
    Tel:400-968-7988    021-33779008
    Monoclonal anti Calbindin D-28k 200ul
    品牌:Swant
    货号:300
    规格:300
    货期:swant

    瑞士swant​公司,瑞士一家生物科技公司,产品包括用于钙结合蛋白(D-28k),钙调蛋白,离子通道蛋白,神经递质的抗体和抗原,用于神经元或神经胶质细胞及癌细胞的染色产品和用于免疫组合的单抗和多抗。多年来专注于钙结合蛋白产品的研究,其产品虽种类虽然不多,但是却都经典热销,并且经常出现在高分的文章中。为国内外众多相关领域科学家所热衷。

    商品详情 参考文献 相关资料

    产品介绍

    品名

    Monoclonal anti Calbindin D-28k 

    货号

    300

    规格

    200ul / 500ul  / 1ml

     克隆性

    monoclonal

    共轭

    nonconjugated

    反应性

    thirteen-lined ground squirrel, Spanish newt, slender lungfish, reedfish , Shaw's jird, gray bichir , African green monkey, human, mouse, rat, chicken, pigs , Xenopus laevis, rhesus macaque

    应用

    western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

    宿主动物

    Mouse monoclonal, IgG1

    抗体形式

    Conc. supernat. lyophil.

    品形式

    Lyophilized concentrated supernatant (no preservatives). 

    复溶

    with 200 µl / 500 µl or 1 ml bidistilled water

    产品描述

    Monoclonal anti-calbindin D-28k is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with calbindin D-28k purified from chicken gut (1). The antibody was evalsuated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labelling as well as Biotin-Avidin labeling of cryostate or vibratome-sections of 4 % paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay. 

    The product is a monoclonal antibody (McAB) against calbindin D-28k, a calcium-binding protein of the EF-hand family related to calmodulin and troponin-C

    稀释

    Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method.

    Immunoblots: 1:100 - 1:1'000 with an indirect peroxidase method. 

    We recommend that the optimal dilutions be determined by titration experiments.

    储存

    For long storage, keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing. 

    参考文献

    1. Celio M.R. et al., Cell Calcium 11:599-602, 1990 

    2. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press 

    3. Garcia-Segura L.M. et al. (1984) Brain Rews. 296: 75-86. 

    4. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493

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